The Effect of Hypochlorous Acid on Cultured Osteoblast and Escherichia Coli

HOCl Trust introduction

Evidence from a Masters student thesis on the potential for HOCl to be used as an effective antiseptic in bone surgery. 

Source

Flaquer Fusté, Cristina, "The Effect of Hypochlorous Acid on Cultured Osteoblast and Escherichia Coli" (2025). MSU Graduate Theses/Dissertations. 4039.

Date: 2025

Abstract

 This study looked at whether HOCl could help prevent infections in bone surgeries and fracture treatments, where infections are often serious and hard to treat. Researchers tested how HOCl affects bone-forming cells (osteoblasts) and the bacteria E. coli, a common cause of bone infection. Osteoblasts were grown in the lab and exposed to different HOCl levels. The cells stayed healthy at 10 ppm and survived 51% at 50 ppm. When tested on E. coli, HOCl at 50 ppm killed more than 99% of the bacteria within minutes. These results suggest that HOCl can effectively kill harmful microbes without severely damaging bone cells. Overall, HOCl appears to be a safe and promising antiseptic for reducing infection risks during orthopedic surgeries and fracture healing.

Link to paper